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Mass Spectrometry of Structurally Modified DNA - Europe PMC Article - Europe PMC
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About this book Nucleic acid research has had a profound impact on molecular biology, resulting in a variety of new disciplines. Mass Spectrometric Analysis of Deoxyinosines. Tandem Mass Spectrometry of Nucleic Acids. Customer Reviews Review this book. Current promotions. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account. If the address matches an existing account you will receive an email with instructions to retrieve your username.
Mass Spectrometry of Nucleosides and Nucleic Acids
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View Preview. In recent years, more and more researchers found both DNA and RNA methylation can be reversed by multiple dioxygenase, which opens a door to further understand mechanism of DNA or RNA methylation and demethylation [ 2 ]. Moreover, 5mdC, as the epigenetic marker of DNA, exerts a predominant role in mammalian genome [ 30 ]. And the important function of 5mdC modification in mammals has attracted much interest in the field of the epigenetic control.
The synthetic methylated DNA substrates were not significantly oxidized in the presence of FTO, and also were not significantly demethylated with the increase of reaction time and dose of FTO in vitro Fig 2d. Nevertheless, we wonder if the decrease of 5mdC level was caused by FTO in vivo. But the mutant TET2 in this article does not have this kind of function.
Hence, the immunofluorescence experiment was implemented and exhibited that excess wild type TET2 instead of FTO caused apparent increasing of cellular 5hmdC levels Fig 3a. We will be more committed to find more regulation of 5mdC modification. On the other hand, we also aim to find out biological effect of FTO in epigenetic modifications.
And these findings will bring an insight into understanding the potential function of FTO in epigenetics and gene expression in higher eukaryotes. We believe that further insight into the functional properties of FTO not only reveals complicated clinical phenomenon, but also may bring expectation to heal many human disease.
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Introduction DNA methylation is an epigenetic modification, which does not change DNA sequence but plays important roles in a variety of cellular processes, including regulation of gene expression, genomic imprinting, X-chromosome inactivation, suppression of transposable elements, maintenance of epigenetic memory, and also important in cell differentiation [ 1 — 6 ].
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Supporting information. S1 Fig. References 1. Bird A. DNA methylation patterns and epigenetic memory. View Article Google Scholar 2.
Annual review of biochemistry. Wu H, Zhang Y. Mechanisms and functions of Tet protein-mediated 5-methylcytosine oxidation. View Article Google Scholar 4. Ehrlich M, Wang RY. The Journal of biological chemistry.
Storb U, Arp B. Methylation patterns of immunoglobulin genes in lymphoid cells: correlation of expression and differentiation with undermethylation. Genome-wide regulation of 5hmC, 5mC, and gene expression by Tet1 hydroxylase in mouse embryonic stem cells.