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Laboratory Methods in Food Microbiology describes a wide range of methods for use in the microbiological analysis of foods, including approaches to counting and detection procedures for microorganisms, identification of bacteria, yeasts and fungi, microbiological analysis for the main food commodity groups, and safety in the food microbiology laboratory.

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Methods for Microbial Detection in Food

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Laboratory methods in food microbiology

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Payment Methods accepted by seller. AbeBooks Bookseller Since: 31 May Home Wilkie F. Stock Image. A preliminary step called enrichment culture may be used to amplify the number of bacterial pathogens, by pre-incubating the food sample in a non-selective medium that promotes growth of any bacteria that might be in the sample. Many standard methods include a two-stage enrichment culture. The first step, or pre-enrichment, involves adding a specific amount determined by weight, typically 10—25 grams of the food to be tested in a large —ml volume of a non-selective broth medium.

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We will be conducting our own investigation of food safety using a modified and scaled down adaptation of the standard laboratory methods, beginning with a pre-enrichment culture of food samples, followed by plated on several types of selective and differential media. Our determination of food contamination will be based on a growth of bacteria on the selective media and b observation of a specific biochemical reaction usually a color change characteristic for a particular type of pathogen.

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Therefore, definitive identification of a bacterium isolated from food requires additional testing. Numerous media formulations are available that permit the isolation and identification of pathogenic bacteria in food. MAC Bile salts and crystal violet inhibits the growth of most Gram positive, non-enteric bacteria.

Lactose and pH indicator Gram negative enteric bacilli will grow; E. Sorbitol MacConkey Agar.

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Sorbitol and pH indicator Gram negative enteric bacilli will grow; E. MSA 7. Pre-enrichment to Promote Bacterial Growth Note : this will require time in addition to your regular lab period to complete.

Because both the presence and type of bacteria that may be in the food is unknown, BSL2 containment practices should be used throughout the entire procedure. Samples of foods will be available in the laboratory up to a week before your scheduled lab period. Transfer 5 ml of Tryptic Soy Broth to a disposable plastic culture tube.

You should select one food sample for testing. Prepare an enrichment culture of the selected food item, by transferring a small amount of the food to the broth in the culture tube, using aseptic technique. A minimum of 18 hours after starting the enrichment culture one day after the enrichment culture is started is preferred , and no later than the day before the scheduled period for this investigation, return to the lab, and use an inoculating loop to subculture samples from the enrichment culture to each of the three types of selective and differential media described in Table 1.

Use the streak plate method for all of the plates, so isolated colonies will form. Appropriately dispose of the enrichment culture as a potential biohazard.

Laboratory Methods in Food Microbiology : W.F. Harrigan :

Return the streak plates to the incubator for observation and further investigation during the lab period. For each of the selective and differential media on which bacterial colonies are observed, indicate what the appearance of the colonies indicates in terms of the possible type s of bacteria present in the food sample. The growth and appearance of colonies on selective and differential media is an indicator of the presence of specific bacterial pathogens, but these results must be confirmed before reporting that food is contaminated and ingestion may initiate a foodborne disease.

Therefore, perform additional tests to confirm that the colonies observed on the selective media are potentially pathogenic bacteria.

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  • These tests include:. Coagulase test to confirm Staphylococcus aureus S.